ERCC1 Gene Expression Analysis Kit
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The ERCC1 test provides information about the potential sensitivity of a patient’s cancer to platinum-based therapies.
The combination of carboplatin and paclitaxel is the most commonly used regimen for the treatment of advanced non-small cell lung cancer (NSCLC) patients. These drugs inhibit cancer growth by damaging DNA. The expression of the DNA-repair gene excision repair cross-complementation group 1 (ERCC1) is reported to be correlated with resistance to platinum-based drugs. Tumor cells with low ERCC1 enzyme may be more sensitive to platinum-based drugs since they are less able to repair damaged DNA.
Results from several clinical studies suggest that a low level of ERCC1 gene expression is a good biomarker for clinical sensitivity to cisplatin, carboplatin, and oxaliplatin. In the IALT Bio study, patients who had non–small-cell lung cancer with no detectable ERCC1 had longer disease-free survival after cisplatin-based chemotherapy than did patients with a tumor that contained ERCC1 (P<0.001) (1).
In a study of ERCC1 gene expression as a prognostic marker in advanced bladder cancer patients receiving cisplatin-based chemotherapy, median survival was significantly higher in patients with low ERCC1 levels (25.4 versus 15.4 months; P = 0.03) (2). And a retrospective study by Ceppi et al of NSCLC found that patients treated with platinum who had low ERCC1 mRNA in their tumors had significantly longer survival than patients with high ERCC1 expression (median survival 14.9 versus 9.9, p = 0.012) (3).
Intended Use: For research use only.
Technological Principles: The Kit is based on a real-time PCR technology called "ADx-ARMS", which uses proprietary primers and probes to quantitate ERCC1 mRNA levels. The probe produces a fluorescent signal when hybridized to the PCR product. Unlike other tests that require post-PCR gel or capillary electrophoresis analysis, the ERCC1 test is a "closed-tube" assay. PCR amplification and signal acquisition occur in a "closed-tube", simplifyng the procedure considerably and avoiding the possibility of carry-over contamination. The assay is sensitive, accurate and produces a robust output (Ct) that is well-suited for quantitative analysis.
AmoyDx ERCC1 Gene Expression Analysis Kit is available in the following size:
|Product Name||Size (test/Kit)||Storage||Cat. No.|
|ERCC1 Gene Expression Analysis Kitt||24t||-20 ° C||ADx-ER01|